期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 103, 期 -, 页码 209-216出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2012.10.018
关键词
Calcium phosphate cement; PLGA; Collagen; Scaffold; Cell response; Plasma treatment; Unidirectional lamellar pore
资金
- National Natural Science Foundation of China (NSFC) [51172074]
- National Basic Research Program of China [2012CB619100]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0919]
In this study, calcium phosphate cement (CPC)-based scaffold with unidirectional lamellar pore structure was fabricated by unidirectional freeze casting. Poly(lactic-co-glycolic acid) (PLGA) was infiltrated into the CPC scaffold to improve its strength and toughness, which compromised the bioactivity and osteoconductivity of CPC. Collagen (Col) was immobilized on the pore surface of the PLGA/CPC scaffold to enhance the bioactivity of the scaffold using plasma treatment under the ammonia (NH3) atmosphere. The immobilization of collagen was characterized by infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Compared to the PLGA/CPC composite scaffold, the Col/PLGA/CPC composite scaffold had higher contact angle, porosity and water absorption, while the compressive strength of both scaffolds was comparable. Rat bone marrow mesenchymal stem cells (rMSCs) seeded on the Col/PLGA/CPC scaffold showed markedly improved cell seeding, attachment, proliferation and differentiation than those on the PLGA/CPC scaffold. These results suggest that the surface immobilization of collagen by plasma treatment can improve the bioactivity of the PLGA/CPC scaffold and the Col/PLGA/CPC composite scaffold is a promising candidate for bone tissue engineering. (c) 2012 Elsevier B.V. All rights reserved.
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