期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 93, 期 -, 页码 24-28出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2011.11.054
关键词
Superparamagnetic; Nanoparticles; Gluconic acid; Lipase
资金
- National Natural Science Foundation of China [21077044]
- Natural Science Foundation of Shandong Province [Y2008B41, ZR2009BM046]
Superparamagnetic magnetite nanoparticles (SMN) were surface-modified with gluconic acid (GLA) to improve their hydrophilicity and bio-affinity. Gluconic acid was successfully coated on the surface of magnetite nanoparticles and characterized using Fourier transform infrared spectroscopy (FT-IR). With water-soluble carbodiimide (EDC) as the coupling reagent, lipase was successfully immobilized onto the hydroxyl-functionalized magnetic nanoparticles. The immobilized lipase had better resistance to temperature and pH inactivation in comparison to the free form and hence widened the reaction pH and temperature range. Thermostability and storage stability of the enzyme improved upon covalent immobilization. Immobilized lipase showed higher activity after recycling when compared to the free one and could be recovered by magnetic separation. (C) 2011 Elsevier B.V. All rights reserved.
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