期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 95, 期 -, 页码 247-253出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2012.03.011
关键词
CdTe QDs; GSH; TGA; Immunolabeling; Colorectal cancer cell imaging
资金
- Natural Science Foundation of China [20875011]
We have successfully synthesized GSH and TGA co-capped CdTe quantum dots (QDs) with good biological compatibility and high fluorescence intensity. The effects of different reaction time, temperature, pH value, ligand concentration and the molar ratio of GSH/TGA were carefully investigated to optimize the synthesis condition. The optical properties of as-prepared CdTe QDs were studied by UV-visible absorption spectrum and fluorescence spectrum, meanwhile their structure and morphology were characterized using transmission electron microscope (TEM), Fourier transform infrared spectra (FT-IR) and X-ray powder diffraction (XRD). Compared with the CdTe QDs that are single-capped with either GSH or TGA, the GSH-TGA co-capped CdTe QDs demonstrated significantly improved fluorescence intensity and optical stability. In addition, GSH-TGA co-capped CdTe QDs were conjugated to amonoclonal antibody ND-1. The GSH-TGA co-capped CdTe QDs-antibody probe was successfully used to label colorectal cancer cells, CCL187, in vitro. (C) 2012 Elsevier B.V. All rights reserved.
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