期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 92, 期 -, 页码 161-167出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2011.11.035
关键词
Cytochrome c; Gold nanoparticle; Atomic force microscopy; Cyclic voltammetry; Biosensor
资金
- Ministry of Education, Science and Technology (MEST) [M10536090001-05N3609-00110]
- Graduate School of Specialization for Biotechnology of Ministry of Knowledge Economy (MKE)
- National Research Foundation of Korea
Here, we describe the formation of a hybrid biointerface consisting of gold nanoparticle (AuNP) and cytochrome c (cyt c) on indium tin oxide (ITO) electrodes using a two-step immobilization procedure. The Au nanoparticles were attached to the ITO electrodes by 3-mercaptopropyl trimethoxysilane (3-MPTMS). The electrode was then incubated with 11-mercapunclecanoic acid (11-MIJA) and the nanoparticles were activated to allow for coupling to cyt c. This process resulted in the formation of the AuNP/cyt c hybrid on the ITO electrode. The ITO/AuNP/cyt c substrate surfaces were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM) and X-ray diffraction analysis (XRD), and cyclic voltammetry (CV) techniques. Further analysis regarding the surface roughness properties of ITO, ITO/AuNP and ITO/AuNP/cyt c were also performed. The ITO/AuNP/cyt c immobilized ITO electrode displayed a pair of well-defined redox peaks (E-pa at 0.09 V and E-pc at 0.02V) at pH 7.0 in HEPES buffer solution. Differential pulse voltammetry (DPV) and amperometric i-t measurements on the modified electrode showed a linear response after the addition of hydrogen peroxide (H2O2). The developed electrode sensor had an electron transfer rate constant (k(s)) of 0.69 s(-1) with a detection limit of 0.5 mu M. The results of this study suggest that the hybrid layers were well fabricated on the ITO surface and the developed ITO/AuNP/cyt c electrode displayed an excellent electrocatalytic response for the detection of H2O2. (C) 2011 Elsevier B.V. All rights reserved.
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