Antibacterial activity and cytocompatibility of chitosan-N-hydroxy-2,3-propyl-N methyl-N,N-diallylammonium methyl sulfate

A water-soluble quaternary ammonium salt of chitosan, chitosan-N-hydroxy-2,3-propyl-N-methyl-N,N-diallylammonium methyl sulfate (MDAACS), was synthesized by reacting chitosan with methyl diallyl ammonium salt (MDAA). The results of water contact angle and swelling ratio showed that the membrane of MDAACS was more hydrophilic than chitosan. The antibacterial activities of MDAACS against Staphylococcus aureus and Klebsiella pneumoniae were evaluated with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The results showed that the antibacterial activity of MDAACS was higher than that of chitosan. The cytocompatibility was evaluated in vitro with L929 fibroblast proliferation based on MTT calorimetric assay. The results showed that cell growth was much higher on MDAACS than on chitosan. (C) 2011 Elsevier B.V. All rights reserved.