期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 87, 期 2, 页码 409-414出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2011.05.052
关键词
Biomolecule immobilization; Printing; Sol-gel; Maleimide; Chimeric avidin; Avidin-biotin technology; Biosensing
资金
- Tekes - the Finnish Funding Agency for Technology and Innovation [BioFace 40055/08]
- Faculty of Science at the University of Oulu
- Oulu University Scholarship Foundation
- Tampere University Hospital
A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol-gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol-gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films. (C) 2011 Elsevier B.V. All rights reserved.
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