期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 82, 期 2, 页码 470-476出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2010.10.004
关键词
Chitosan; Atomic force microscopy; Single molecule force spectroscopy; Biopolymer desorption
资金
- NSERC
- FQRNT
- CFI
- Concordia University
- Sigma Xi
Atomic force microscopy (AFM) and AFM-based force spectroscopy was used to study the desorption of individual chitosan polymer chains from substrates with varying chemical composition. AFM images of chitosan adsorbed onto a flat mica substrate show elongated single strands or aggregated bundles. The aggregated state of the polymer is consistent with the high level of flexibility and mobility expected for a highly positively charged polymer strand. Conversely, the visualization of elongated strands indicated the presence of stabilizing interactions with the substrate. Surfaces with varying chemical composition (glass, self-assembled monolayer of mercaptoundecanoic acid/decanethiol and polytetrafluoroethylene (PTFE)) were probed with chitosan modified AFM tips and the corresponding desorption energies, calculated from plateau-like features, were attributed to the desorption of individual polymer strands. Desorption energies of 2.0 +/- 0.3 x 10(-20) J, 1.8 +/- 0.3 x 10(-20) J and 3.5 +/- 0.3 x 10(-20) J were obtained for glass, SAM of mercaptoundecanoic/dodecanethiol and PTFE, respectively. These single molecule level results can be used as a basis for investigating chitosan and chitosan-based materials for biomaterial applications. (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据