期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 83, 期 1, 页码 83-90出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2010.11.001
关键词
N,N '-Carbonyldiimidazole; Superparamagnetic nanoparticles; Magnetite; Particulate vaccine carrier
资金
- Australian Research Council [DP0986357]
- Australian Research Council [DP0986357] Funding Source: Australian Research Council
Particulates with specific sizes and characteristics can induce potent immune responses by promoting antigen uptake of appropriate immuno-stimulatory cell types. Magnetite (Fe3O4) nanoparticles have shown many potential bioapplications due to their biocompatibility and special characteristics. Here, superpairamagnetic Fe3O4 nanoparticles (SPIONs) with high magnetization value (70 emu g(-1)) were stabilized with trisodium citrate and successfully conjugated with a model antigen (ovalbumin, OVA) via N,N'-carbonyldiimidazole (CDI) mediated reaction, to achieve a maximum conjugation capacity at approximately 13 mu g mu m(-2). It was shown that different mechanisms governed the interactions between the OVA molecules and magnetite nanoparticles at different pH conditions. We evaluated as-synthesized SPION against commercially available magnetite nanoparticles. The cytotoxicity of these nanoparticles was investigated using mammalian cells. The reported CDI-mediated reaction can be considered as a potential approach in conjugating biomolecules onto magnetite or other biodegradable nanoparticles for vaccine delivery. (C) 2010 Elsevier B.V. All rights reserved.
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