期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 76, 期 1, 页码 298-304出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2009.11.008
关键词
Biosurfactant; Lactobacillus paracasei; Critical micelle concentration; Antimicrobial; Anti-adhesive; Autoaggregation
资金
- FCT (Portugal) [PTDC/SAU-BEB/73498/2006]
- Fundação para a Ciência e a Tecnologia [PTDC/SAU-BEB/73498/2006] Funding Source: FCT
In this study, the crude biosurfactant produced by a Lactobacillus paracasei strain isolated in a Portuguese dairy industry was characterized. The minimum surface tension (41.8 mN/m) and the critical micelle concentration (2.5 mg/ml) obtained were found to be similar to the values previously reported for biosurfactants isolated from other lactobacilli. The biosurfactant was found to be stable to pH changes over a range from 6 to 10, being More effective at pH 7, and showed no loss of surface activity after incubation at 60 degrees C for 120 h. Although the biosurfactant chemical composition has not been determined yet, a fraction was isolated through acidic precipitation, which exhibited higher surface activity as compared with the crude biosurfactant. Furthermore, this isolated biosurfactant showed antimicrobial and anti-adhesive activities against several pathogenic microorganisms. In addition, L. paracasei exhibited a strong autoaggregating phenotype, which was maintained after washing and resuspending the cells in PBS, meaning that this attribute must be related to cell surface components and not to excreted factors. The autoaggregation ability exhibited by this strain, together with the antimicrobial and anti-adhesive properties observed for this biosurfactant opens the possibility for its use as an effective probiotic strain. (C) 2009 Elsevier B.V. All rights reserved.
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