期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 81, 期 2, 页码 600-606出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2010.08.007
关键词
Core/shell structure; Fe3O4/Au nanocomposites; Surface plasmon resonance (SPR); Human IgM
资金
- National Natural Science Foundation of China [20727003]
- Science and Technology Developing Foundation of Jilin Province [20070551]
In this paper, the core/shell Fe3O4/Au nanocomposites modified with 3-mercaptopropionic acid (MPA) were prepared and applied in the surface plasmon resonance (SPR) biosensor. And the detailed investigation of Fe3O4/Au nanocomposites was separately performed by UV-vis spectroscopy and transmission electronic microscopy. As the magnetic property and exceptional optical properties, the Fe3O4/Au nanocomposites were used as the solid support for the goat anti-human IgM, which could be immobilized on the surface of SPR biosensor chip by a magnetic pillar. This novel method of immobilizing goat anti-human IgM simplified experimental procedures and facilitated the regeneration of the sensing membrane. In addition, the different diameter of Fe3O4/Au nanocomposites could be obtained with the different amount of MPA in the solution. And the effect of Fe3O4/Au nanocomposites with different diameters on the sensitivity of SPR biosensor was also explored. As a result, the SPR biosensor exhibits a satisfactory response for human IgM in the concentration range of 0.30-20.00 mu g ml(-1) and the increasing nanocomposite diameter is in favor of the sensitivity enhancement of SPR biosensor. (C) 2010 Elsevier B.V. All rights reserved.
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