期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 81, 期 2, 页码 389-396出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2010.07.024
关键词
Blood compatibility; Hirudin; PEG; Gold; Protein resistant surface; Antithrombin
资金
- NSERC (Canada)
- Canadian Institutes of Health Research (CIHR)
- NSERC (PGS/D)
In this work, we hypothesize that a surface modified with both polyethylene glycol (PEG) and hirudin may provide a non-fouling, thrombin-neutralizing surface suitable for blood contacting applications. With gold as a model substrate we used two different approaches to the preparation of such a surface: (1) a direct method in which PEG was conjugated to hirudin and the conjugate was then immobilized on the gold; (2) a sequential method in which PEG was immobilized on the gold and hirudin then attached to the immobilized PEG. The surfaces were characterized by water contact angle, ellipsometry and XPS. The biological properties were investigated by measuring protein adsorption (fibrinogen and thrombin) from buffer and plasma; thrombin inhibition was measured using a chromogenic substrate assay. Hirudin immobilization was found to be more efficient on surfaces prepared by the direct method. Sequential surfaces, however, despite having a lower density of hirudin, showed greater biological activity (thrombin binding and inhibition). (C) 2010 Elsevier B.V. All rights reserved.
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