Protein adsorption and cell adhesion on polyurethane/Pluronic (R) surface with lotus leaf-like topography

Lotus leaf-like polyurethane/Pluronic (R) F-127 surface was fabricated via replica molding using a natural lotus leaf as the template. Water contact angle measurements showed that both the hydrophobicity of the unmodified polyurethane (PU) surface and the hydrophilicity of the PU/Pluronic (R) surface were enhanced by the construction of lotus leaf-like topography. Protein adsorption on the PU/Pluronic (R) surface without topographic modification was significantly lower than on the PU surface. Adsorption was further reduced when lotus leaf-like topography was constructed on the PU/Pluronic (R) surface. Cell culture experiments with L929 cells showed that adhesion on the PU/Pluronic (R) surface with lotus leaf-like topography was low and adherent cells were spherical and of low viability. The PU/Pluronic (R) surface with lotus leaf-like topography thus appears to be resistant to nonspecific protein adsorption and to cell adhesion, and these effects derive from the both chemical composition and topography. The results suggest a new strategy based on surface topography for the design of antifouling materials. (C) 2010 Elsevier B.V. All rights reserved.