Effect of cytoskeleton inhibitors on deadhesion kinetics of HepG2 cells on biomimetic surface

Cytochalasin-D (Cyto-D) and latrunculin-A (Lit-A) are known inhibitors of actin microfilaments and adversely affect the physiological functions of anchorage-dependent cells Alternatively, doxorubicin (pox). a chemotherapeutic ding is known to induce apoptosis and cell detachment of tumor cells However, the intricate inter play between drug administration, cytoskeletal rearrangement and biophysical responses of live cells on immobilized layer of exit acellular matrix (ECM) protein remains unknown In this study, the deadhesion kinetics and actin remodeling of live HepG2 cells following the addition oldie three drugs are probed with confocal reflectance interference contrast microscopy (C-RICM) and fluorescence confocal microscopy First, it is shown that the reduction in two-dimensional spread area of HepG2 cells is 10 5%. 15.4% and 21 9% under the influence of 5 mu M of Lat-A. Cyto-D and Dox, respectively Secondly. C-RICM demonstrates the recession of strong adhesion contact against time of cell seeding upon the addition of the three drugs Thirdly, the initial cell detachment rate and extent of reduction in the degree of cell deformation (a/R) are dependent on both the drug types and concentration. Lastly, oscillation-like responses of a/R and adhesion energy are uniquely found in Lit-A induced cell detachment Overall. our biophysical approaches have been pi oven as a highly quantitative platform for elucidating the interfacial properties of adherent cells on biomimetic surfaces under cytoskeleton disruption (C) 2009 Elsevier B V All rights reserved