In vitro cyto-biocompatibility and cell detachment of temperature-sensitive dextran hydrogel

A series of poly(NIPAAm-co-GMA-Dex) hydrogels were synthesized from glycidyl methacrylate-derivatized dextran (GMA-Dex) and N-isopropylacrylamide (NIPAAm) in aqueous solution. The swelling properties, cell culture and detachment behaviors of these hydrogels were investigated. It has found that poly(NIPAAm-co-GMA-Dex) hydrogels are not only sensitive to temperature in deionized water and Dulbecco's modified eagle medium (DMEM) (10% FBS), but also exhibit good biocompatibility. In addition, L929 cells were found to detach from the hydrogel surface naturally without affecting the function of L929 by controlling environmental temperature. (C) 2009 Elsevier B.V. All rights reserved.