期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 70, 期 2, 页码 181-186出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2008.12.018
关键词
Chitosan; Galactose; Non-viral vector; DNA; Gene delivery
资金
- Foundation of Education Ministry of China [20070610053]
- Sichuan Province [2006Z06-010]
It is important for gene carrier to transport DNA into target cells. Although viral vectors are very efficient gene-transfer vehicles, significant drawbacks limit their applications. Chitosan (CS) has been researched widely as a non-viral vector. However, the low cell specificity and low transfection efficiency of chitosan need to be overcome. In order to conquer the drawback of chitosan, the present paper is concerned with the synthesis of novel galactosylated chitosan (GC) through etherization of chitosan and galactose in THF using BF3 center dot OEt2 as promoter. The final product was characterized and confirmed by FT-IR and H-1 NMR. The degree of O-substitution (DS) of chitosan by galactose was measured to be 10.38% using anthrone-sulfuric acid colorimetric method. The mean particle diameter and average zeta potential of the GC/DNA complex were 350 nm and +22.1 mV, respectively. The GC/DNA nanoparticle was tested to transfect HEK293 cells, and the viability of HEK293 cells was not affected by the GC/DNA nanoparticle compared to that of the control. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.
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