期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 65, 期 2, 页码 190-196出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2008.04.001
关键词
hemoglobin; TiO2 colloid; UV/vis spectroscopy; fluorescence spectroscopy; binding constant
The interaction between bovine hemoglobin (BHb) and TiO2 colloid was investigated by UV/vis absorption, UV/vis diffuse reflectance spectrum, IR, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques under physiological pH 7.40. TiO2 effectively quenched the intrinsic fluorescence of BHb via static quenching. The process of binding TiO2 on BHb was a spontaneous molecular interaction procedure. The thermodynamic parameters, Delta H degrees and Delta S degrees, were estimated to be -78.07 kJ mol(-1), -110.93 J mol(-1) K-1 according to the van' Hoff equation. This indicates that the van der Waals and hydrogen bonds interactions played a major role in stabilizing the TiO2-BHb complex. The effect of TiO2 on the conformation of BHb was analyzed using synchronous fluorescence spectroscopy. (C) 2008 Published by Elsevier B.V.
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