期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 63, 期 2, 页码 254-261出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2007.12.007
关键词
gold nanoparticles; poly-o-phenylenediamine; surface plasmon resonance; electrochemistry; immunoassay
The poly-o-phenylenediamine (PoPD) nonconducting film and gold nanoparticles (AuNPS) were combined to fabricate AuNPs/PoPD film, which is used as a novel biocompatible interface for the immobilization of antibody and develop a simple and sensitive label-free immunoassay for the detection of the related antigen (human immunoglobulin G (IgG)). Surface plasmon resonance (SPR) and electrochemical methods were used to provide the real-time information about the polymer film growth, assembling of various sizes of gold nanoparticles, anti-human IgG antibody (anti-hIgG) immobilization and the antigen-antibody interaction. The microstructures of the PoPD and AuNPs/PoPD films were characterized by atomic force microscopy (AFM). These results demonstrated that AuNPs were uniformly dispersed on the porous surface of PoPD film, which formed a nano-structure biocompatible AuNPs/PoPD interface. The use of gold nanoparticles and PoPD film could enhance the immunoassay sensitivity and anti-nonspecific property of the resulting immunoassay electrode. Additionally, the reproducibility and preliminary application of anti-hIgG/AuNPs/PoPD/Au electrode for SPR detection of hIgG was also evaluated. (C) 2008 Published by Elsevier B.V.
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