期刊
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
卷 452, 期 -, 页码 115-124出版社
ELSEVIER
DOI: 10.1016/j.colsurfa.2014.03.085
关键词
PLGA; Smooth microsphere; Porosity; 3D culture; Ovarian cancer cells
资金
- National Basic Research Program of China [2011CB933503]
- program for New Century Excellent Talents in University [NCET-09-0298]
- Special Project on Development of National Key Scientific Instruments and Equipment of China [2011YQ03013403]
- Suzhou Applied Basic Research Fund [SYG201003]
In this study, we prepared surface-smooth and porous poly(lactic-co-glycolic acid) (PLGA) microspheres with different diameters, different sizes of pores and different densities of pores using a simple oil/water emulsion method and varying the preparation conditions, including the molecular weight of PLGA, the components of the PLGA and the addition of porogen. The surface-smooth nonporous and porous PLGA microspheres were also further modified with collagen I. The characteristics of these PLGA microspheres, including original and collagen I-coated PLGA microspheres, were evaluated in a three-dimensional (3D) culture of ovarian cancer HO8910 cells. The HO8910 cells can growth better on original porous and collagen I-coated PLGA microspheres, and express remarkably E-cadherin. These results indicate that porous PLGA microspheres and collagen-coated PLGA microspheres are promising candidates as ovarian cancer cell culture microcarriers for pathological study and high-throughput antitumor drug screening. (C) 2014 Elsevier B.V. All rights reserved.
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