期刊
ACS NANO
卷 9, 期 5, 页码 5609-5617出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b01954
关键词
DNA nanostructures; biomimicry; nanotechnology; RNA detection; live cell imaging
类别
资金
- NUS Engineering-Medicine Cross-Faculty grant [R279000414112]
- Lee Kuan Yew Postdoctoral Fellowship
Rapid and precise in situ detection of gene expressions within a single cell is highly informative and offers valuable insights into its state. Detecting mRNA within single cells in real time and nondestructively remains an important challenge. Using DNA nanotechnology and inspired by nature's many examples of protective-yet-accessible exoskeletons, we designed our mRNA nanosensor, nano-snail-inspired nucleic acid locator (nano-SNEL), to illustrate these elements. The design of the nano-SNEL is composed of a sensory molecular beacon module to detect mRNA and a DNA nanoshell component, mimicking the functional anatomy of a snail. Accurate and sensitive visualization of mRNA is achieved by the exceptional protection conferred by the nanoshell to the sensory component from nucleases-mediated degradation by approximately 9-25-fold compared to its unprotected counterpart. Our nano-SNEL design strategy improved cell internalization is a demonstration of accurate, dynamic spatiotemporal resolved detection of RNA transcripts in living cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据