期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 47, 页码 14053-14056出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201508635
关键词
Alzheimer's disease; biosensors; brain chemistry; copper ions; L-cysteine
资金
- NSFC [21175098, 51263021, 21205102, 21305104]
- National Nature Science Fund for distinguished young scholars [21325521]
- China Postdoctoral Science Foundation [2014M561436]
Copper ion (Cu2+) and l-cysteine (CySH) are closely correlated with physiological and pathological events of Alzheimer's Disease (AD), however the detailed mechanism is still unclear, mainly owing to a lack of accurate analytical methods in live brains. Herein, we report a single biosensor for electrochemical ratiometric detection of Cu2+ and CySH in live rat brains with AD. N,N-di-(2-picoly)ethylenediamine (DPEA) is first synthesized for specific recognition of Cu2+ to form a DPEA-Cu2+ complex. This complex shows high selectivity for CySH owing to the release of Cu2+ from the complex through CySH binding to Cu2+ center. In parallel, 5'-MB-GGCGCGATTTTTTTTTTTTT-SH-3' (HS-DNA-MB, MB = Methylene Blue) is designed as an inner-reference for providing a built-in correction to improve the accuracy. As a result, combined with the amplified effect of Au nanoleaves, our single ratiometric biosensor can be successfully applied in real-time detection of Cu2+ and CySH in the live rat brains with AD. To our knowledge, this is the first report on the accurate concentrations of Cu2+ and CySH in live rat brains with AD.
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