期刊
CLINICAL ORAL IMPLANTS RESEARCH
卷 25, 期 2, 页码 E133-E139出版社
WILEY-BLACKWELL
DOI: 10.1111/clr.12075
关键词
biomaterials; dendrons; mesenchymal stem cells; phosphoserine; Wnt; beta-catenin
资金
- Local FIL funds from the University of Parma
- Regione Emilia Romagna
- Fondazione Cariparma
ObjectivesPhosphoserine-based functionalization has been proposed as a tool to improve integration of endosseous implants by promoting osteoblast adhesion and differentiation in vitro. The present work investigates whether phosphoserine-tethered poly(epsilon-lysine) dendrons, when applied as a film to titanium surfaces, enhance the differentiation of osteoblastic cells and the activation of Wnt/-catenin signaling. Materials and methodsThese films were tested in a murine model of calvaria-derived MC3T3 osteoblastic cells, primary bone marrow cells and mesenchymal, undifferentiated C2C12 cells. Gene expression was assayed by Real Time PCR, and activation of Wnt signaling pathway was measured with a reporter assay. ResultsDendrons increased expression of alkaline phosphatase and osteocalcin, two osteoblastic markers, in both murine osteoblastic MC3T3 cells and primary bone marrow cells. The expression of osteoprotegerin, a protein opposing osteoclastogenesis was also significantly higher in cells growing on dendron-coated substrates both at 3 and 6days of culture. Similarly, the mRNA levels of Wisp-2 and of -catenin, two Wnt target genes, were also markedly increased in this group at day 6. The activation of this signaling pathway in cells growing on the dendron-coated surfaces was confirmed by use of a TCF/-catenin reporter system in the C2C12 cell line. ConclusionsThe findings of the present study show that phosphoserine-tethered poly(epsilon-lysine) dendron films act as stimuli for the activation of specific signal cascades and promote the differentiation of adhering progenitor cells into an osteoblastic phenotype.
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