期刊
JOURNAL OF MASS SPECTROMETRY
卷 50, 期 6, 页码 812-819出版社
WILEY
DOI: 10.1002/jms.3589
关键词
hydrogen/deuterium exchange; allergen; FTMS; protein complex
资金
- USDA [2011-67017-20079]
- NSF Division of Materials Research [DMR-11-57490]
- State of Florida
The potential epitope of a recombinant food allergen protein, cashew Ana o 1, reactive to monoclonal antibody, mAb 2G4, has been mapped by solution-phase amide backbone H/D exchange (HDX) monitored by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Purified mAb 2G4 was incubated with recombinant Ana o 1 (rAna o 1) to form antigen: monoclonal antibody (Ag: mAb) complexes. Complexed and uncomplexed (free) rAna o 1 were then subjected to HDX-MS analysis. Five regions protected from H/D exchange upon mAb binding are identified as potential conformational epitope-contributing segments. Copyright (C) 2015 John Wiley & Sons, Ltd.
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