期刊
JOURNAL OF MASS SPECTROMETRY
卷 50, 期 6, 页码 854-863出版社
WILEY-BLACKWELL
DOI: 10.1002/jms.3596
关键词
tyramine-based hyaluronan derivatives; isomer discrimination; ion mobility; mass spectrometry; nuclear magnetic resonance spectroscopy
资金
- Czech Science Foundation [P206/12/1150]
- Ministry of Education, Youth and Sports of the Czech Republic [LO1305]
- Operational Program 'Education for Competitiveness' [CZ.1.07/2.3.00/30.0004]
Hyaluronic acid is a naturally occurring linear polysaccharide with substantial medical potential. In this work, discrimination of tyramine-based hyaluronan derivatives was accessed by ion mobility-mass spectrometry of deprotonated molecules and nuclear magnetic resonance spectroscopy. As the product ion mass spectra did not allow for direct isomer discrimination in mixture, the reductive labeling of oligosaccharides as well as stable isotope labeling was performed. The ion mobility separation of parent ions together with the characteristic fragmentation for reduced isomers providing unique product ions allowed us to identify isomers present in a mixture and determine their mutual isomeric ratio. The determination used simple recalculation of arrival time distribution areas of unique ions to areas of deprotonated molecules. Mass spectrometry data were confirmed by nuclear magnetic resonance spectroscopy. Copyright (C) 2015 John Wiley & Sons, Ltd.
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