期刊
CLINICAL MICROBIOLOGY AND INFECTION
卷 17, 期 2, 页码 241-244出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1469-0691.2010.03246.x
关键词
Emerging infectious diseases; high-throughput sequencing technology; novel influenza A (H1N1) virus; Solexa system; viral detection
资金
- 'AIDS and viral hepatitis and other major infectious diseases prevention and control' technology projects [2009ZX10004-102]
- National Basic Research Program of China [2010CB534003, 2005CB522905]
- Institute of Pathogen Biology [2008IPB010]
P>The detection of emerging infectious diseases has been a continuing concern, especially with the novel influenza A (H1N1) viral pandemic of 2009. In the present study, we validated a 'second-generation' parallel sequencing platform for viral detection in swab samples collected during recent influenza virus infections in Beijing. This operation yielded millions of valid reads per sample and resulted in an almost complete spectrum of nucleotide information. Importantly, novel A (H1N1) and seasonal A (H3N2) influenza virus-derived sequences were detected without prior knowledge or use of genetic information in advance, suggesting that this approach could be a valuable tool for diagnosing emerging infectious diseases.
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