期刊
CLINICAL MICROBIOLOGY AND INFECTION
卷 14, 期 11, 页码 1075-1079出版社
ELSEVIER SCI LTD
DOI: 10.1111/j.1469-0691.2008.02083.x
关键词
16S rRNA; groEL; oral cavity; reservoir; Streptococcus sinensis
资金
- University Development Fund
- Committee for Research and Conference Grant,
- Outstanding Young Researcher Award
- HKU Special Research Achievement Award
- The Croucher Senior Medical Research Fellowship
Using Streptococcus sinensis 16S rRNA-specific and groEL gene-specific primers, a 128-bp fragment of the 16S rRNA gene and a 433-bp fragment of the groEL gene were amplified from bacterial DNA recovered from 22 of 100 saliva samples from healthy volunteers. There was no nucleotide difference between the 88-bp 16S rRNA gene fragments from the 22 saliva samples and that of S. sinensis strain HKU4(T), but there were zero to eight nucleotide differences between the 311-bp groEL gene fragments from the 22 samples and that of S. sinensis strain HKU4(T). The oral cavity is the natural reservoir of S. sinensis.
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