期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 6, 页码 2002-2006出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201508104
关键词
affinity-based probes; bioorthogonality; imaging; photo-crosslinking; tetrazoles
资金
- National Medical Research Council [CBRG/0038/2013]
- Ministry of Education of Singapore [MOE2012-T2-1-116, MOE2012-T2-2-051]
The bioorthogonality of tetrazole photoclick chemistry has been reassessed. Upon photolysis of a tetrazole, the highly reactive nitrile imine formed undergoes rapid nucleophilic reaction with a variety of nucleophiles present in a biological system, along with the expected cycloaddition with alkenes. The alternative use of the tetrazole photoclick reaction was thus explored: tetrazoles were incorporated into Bodipy and Acedan dyes, providing novel photo-crosslinkers with one- and two-photon fluorescence Turn-ON properties that may be developed into protein-detecting biosensors. Further introduction of these photo-activatable, fluorogenic moieties into staurosporine resulted in the corresponding probes capable of photoinduced, no-wash imaging of endogenous kinase activities in live mammalian cells.
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