期刊
CLINICAL INFECTIOUS DISEASES
卷 56, 期 11, 页码 1589-1600出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cid/cit127
关键词
C. difficile; mortality; biomarkers; strain-specific variation
资金
- National Institute for Health Research (NIHR)
- UKCRC Modernising Medical Microbiology Consortium
- UKCRC Translational Infection Research Initiative
- Medical Research Council
- Biotechnology and Biological Sciences Research Council
- National Institute for Health Research on behalf of the Department of Health [G0800778]
- Wellcome Trust [087646/Z/08/Z]
- Optimer Pharmaceuticals
- bioMerieux
- Optimer
- Novacta
- Pfizer
- Summit
- Medicines Company
- Viropharma
- MRC [G0800778] Funding Source: UKRI
- Medical Research Council [G0800778] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0512-10047, DRF-2010-03-40, NF-SI-0508-10279] Funding Source: researchfish
Background. Despite substantial interest in biomarkers, their impact on clinical outcomes and variation with bacterial strain has rarely been explored using integrated databases. Methods. From September 2006 to May 2011, strains isolated from Clostridium difficile toxin enzyme immunoassay (EIA)-positive fecal samples from Oxfordshire, United Kingdom (approximately 600 000 people) underwent multilocus sequence typing. Fourteen-day mortality and levels of 15 baseline biomarkers were compared between consecutive C. difficile infections (CDIs) from different clades/sequence types (STs) and EIA-negative controls using Cox and normal regression adjusted for demographic/clinical factors. Results. Fourteen-day mortality was 13% in 2222 adults with 2745 EIA-positive samples (median, 78 years) vs 5% in 20 722 adults with 27 550 EIA-negative samples (median, 74 years) (absolute attributable mortality, 7.7%; 95% CI, 6.4%-9.0%). Mortality was highest in clade 5 CDIs (25% [16 of 63]; polymerase chain reaction (PCR) ribotype 078/ST 11), then clade 2 (20% [111 of 560]; 99% PCR ribotype 027/ST 1) versus clade 1 (12% [137 of 1168]; adjusted P <.0001). Within clade 1, 14-day mortality was only 4% (3 of 84) in ST 44 (PCR ribotype 015) (adjusted P =.05 vs other clade 1). Mean baseline neutrophil counts also varied significantly by genotype: 12.4, 11.6, and 9.5 x 10(9) neutrophils/L for clades 5, 2 and 1, respectively, vs 7.0 x 10(9) neutrophils/L in EIA-negative controls (P <.0001) and 7.9 x 10(9) neutrophils/L in ST 44 (P =.08). There were strong associations between C. difficile-type-specific effects on mortality and neutrophil/white cell counts (rho = 0.48), C-reactive-protein (rho = 0.43), eosinophil counts (rho = -0.45), and serum albumin (rho = -0.47). Biomarkers predicted 30%-40% of clade-specific mortality differences. Conclusions. C. difficile genotype predicts mortality, and excess mortality correlates with genotype-specific changes in biomarkers, strongly implicating inflammatory pathways as a major influence on poor outcome after CDI. PCR ribotype 078/ST 11 (clade 5) leads to severe CDI; thus ongoing surveillance remains essential.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据