期刊
CLINICAL INFECTIOUS DISEASES
卷 54, 期 -, 页码 S273-S279出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cid/cir982
关键词
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资金
- Bill & Melinda Gates Foundation [38180]
- National Institutes of Health, National Institute of Allergy and Infectious Disease, Division of AIDS [NO1-AI-5004, NIAID K23 AI074423-05]
In resource-limited settings, there is increased demand for human immunodeficiency virus type 1 drug resistance testing. Because preservation of plasma specimens is often not feasible in resource-limited settings, use of dried blood spots (DBSs) is being adopted. We used 2 panels of DBSs for genotyping assay validation and proficiency testing in selected laboratories in the World Health Organization laboratory network in 14 countries. An amplification sensitivity of 1000 copies/mL was achieved by 2 laboratories. Reproducibility and accuracy of nucleotide sequence determination and resistance-associated mutation identification from DBSs was similar to that previously determined for plasma. International shipping at ambient temperature had no significant effect on amplification success. These studies indicate that DBS-based genotyping is equally reproducible and reliable, although slightly less sensitive, compared with plasma.
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