期刊
CLINICAL IMMUNOLOGY
卷 153, 期 1, 页码 211-219出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2014.04.016
关键词
Multiple sclerosis; Plasmocytoid dendritic cells; Toll-like receptor; Type I interferon; Immunoregulation; Autoimmunity
类别
资金
- Polish German [P-N/009/2006]
- Polish National Science Center MAESTRO Grant [2012 /04/A/NZ6/00423]
- Polish Swiss Research Programme [PSPB-007/2010]
- European Research Area Network (ERA.Net) [RUS/04/2012]
- Polish National Science Center Grant [2011/01/N/NZ4/04962, 2011/01/N/NZ6/01849]
Plasmacytoid dendritic cells (pDCs), an important immunoregulatory population, are characterized by vigorous secretion of type I interferons (IFNs) in response to toll-like receptor (TLR) 7 and 9 stimulation. We studied the function of pDCs in multiple sclerosis (MS) patients by analysis of TLR7 responses. We assessed a pDC secretion pattern of cytokines in the short term PBMC cultures stimulated with TLR7 agonist. pDCs sorted from PBMCs of both MS patients and controls were used to assess TLR7 expression profile. TLR7 induced signaling in pDCs has been analyzed with intracellular flow cytometry. We have identified a clinically correlated significant decrease of the TLR7-induced IFN-alfa (IFNa) secretion by pDCs from MS patients. This deficit has been accompanied by insufficient intracellular phosphorylation of protein kinase Akt and a decrease of the TLR7 gene expression in MS pDCs. Our results demonstrated a selective pDC deficit in MS supporting a relationship between pDCs and mechanisms of MS. (C) 2014 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据