期刊
CLINICAL IMMUNOLOGY
卷 142, 期 3, 页码 232-236出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2011.12.013
关键词
Cigarette smoke; Influenza; Gamma delta T cells; Nasal mucosa
类别
资金
- NHLBI NIH HHS [R01 HL095163-03, R01 HL095163] Funding Source: Medline
- NIEHS NIH HHS [T32 ES007126, P30 ES010126] Funding Source: Medline
Smokers are more susceptible to respiratory infections, including influenza. To explore the effect of smoking on influenza-induced responses within the nasal mucosa, we have developed a protocol using inoculation with live attenuated influenza virus (LAIV) vaccine followed by sampling of the nasal mucosa. Mucosal cell populations were harvested through superficial biopsy of the nasal inferior turbinate pre and post LAIV inoculation and analyzed using flow cytometry. The majority of nasal biopsy CD45+ immune cells at baseline were CD3+ T lymphocytes. Following LAIV, these lymphocytes increased in nonsmokers but not in smokers. A subset of individuals was negative for helper T cell marker CD4 and cytotoxic T cell marker CD8 but positive for the gamma delta T cell receptor (TCR). Increases in gamma delta TCR+ cells were greater in nonsmokers, than in smokers. Thus, LAIV-induced changes in CD3 T as well as gamma delta T lymphocyte percentages are suppressed in smokers compared to nonsmokers. (C) 2012 Elsevier Inc. All rights reserved.
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