期刊
CLINICAL IMMUNOLOGY
卷 138, 期 1, 页码 60-66出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2010.09.008
关键词
Acute anterior uveitis; Heterogeneous nuclear ribonucleoprotein H3; Hnrph3; Autoantigen; ORF phage display; Disease biomarker
类别
资金
- NIH [R01EY016211, R01EY016211-05S1, P30-EY014801]
- Research to Prevent Blindness
- NATIONAL EYE INSTITUTE [R01EY016211, P30EY014801] Funding Source: NIH RePORTER
Acute anterior uveitis (AAU) is the most common form of autoimmune uveitis in the eye with few known autoantigens. Identification of autoantigens will improve our understanding of the molecular mechanisms and capability for disease diagnosis. Phage display is a powerful technology for autoantigen identification. However, because of uncontrollable reading frames, phage display with conventional cDNA libraries identifies high percentage of non-open reading frames (non-ORFs) with minimal implications for autoantigen identification. We recently developed ORF phage display technology with minimal reading frame problem. Herein we used ORE phage display to identify 18 patient-specific clones, including 16 ORFs encoding endogenous proteins as candidate autoantigens for AAU. One of the identified antigens was heterogeneous nuclear ribonucleoprotein H3 (Hnrph3) that was further characterized for MU relevance and independently verified by Western blot. These results demonstrate that ORE phage display is a valuable approach for identification of unknown autoantigens. (C) 2010 Elsevier Inc. All rights reserved.
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