期刊
CLINICAL IMMUNOLOGY
卷 131, 期 2, 页码 260-270出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2008.12.001
关键词
GM-CSF; Dendritic cells; Regulatory T cells; Tolerance; Type 1 diabetes; NOD
类别
资金
- National Institutes of Health Health [R01 A1058190, R21 A1069848]
Abnormalities in DC function are implicated in defective immune regulation that leads to type-1 diabetes (T1D) in NOD mice and humans. In this study, we used GM-CSF and Flt3-L to modulate DC function in NOD mice and observed the effects on T1D development. Treatment with either ligand at earlier stages of insulitis suppressed the development of T1D. Unlike Flt3-L, GM-CSF was more effective in suppressing T1D, even when administered at later stages of insulitis. In vitro studies and in vivo adoptive transfer experiments revealed that CD4+CD25+ T cells from GM-CSF-treated mice could suppress effector Tcell response and T1D. This suppression is likely mediated through enhanced IL-10 and TGF-beta 1 production. Adoptive transfer of GM-CSF exposed DCs to naive mice resulted in an expansion of Foxp3+ Tcells and a significant delay in T1D onset. Our results indicate that GM-CSF acted primarily on DCs and caused an expansion of Foxp3+ Tregs which delayed the onset of T1D in NOD mice. (C) 2008 Elsevier Inc. All rights reserved.
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