Targeted disruption of the galectin-3 gene results in decreased susceptibility to multiple low dose streptozotocin-induced diabetes in mice

Galectin 3 (Gal-3) is an antiapoptotic and a proinflammatory lectin. We hypothesized that the proinflarnmatory properties of Gal-3 may influence disease induction in the multiple low doses of streptozotocin model. of diabetes. Diabetes was induced in C57BL/6 Gal-3(+/+) and Gal-3(-/-) mice and disease monitored by blood glucose level, immuno-histology, insulin content of islets and expression of the proinflarnmatory cytokines, TNF-alpha, IFM-gamma, IL-17, and iNOS in pancreatic lymph nodes. Gat-3(+/+) mice developed delayed and sustained hyperglycemia, mononuclear cellular infiltration and reduced insulin content of islets accompanied with expression of proinflammatory cytokines. Gal-3(-)/(-) mice were relatively resistant to diabetogenesis as evaluated by glycemia, quantitative histology and insulin content. Further, we observed the weaker expression of IFN-gamma and complete absence of TNF-a, and IL-17 in draining pancreatic lymph nodes. Macrophages, the first cells that infiltrate the islet in this model. of diabetes, produce less TNF-alpha and NO in Gal-3(- /) mice. Thus, Gal-3 is involved in immune mediated beta cell damage and is required for diabetogenesis in this model of disease. (c) 2008 Published by Elsevier Inc.