期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 39, 页码 11419-11423出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201503640
关键词
aggregation-induced emission; endo/lysosomes; gene delivery; polymers; reactive oxygen species
资金
- SMART [R279-000-378-592]
- Ministry of Education [R279-000-391-112]
- Singapore NRF Investigatorship [R279-000-444-281]
- Institute of Materials Research and Engineering of Singapore [IMRE/14-8P1110]
Endo/lysosomal escape of gene vectors and the subsequent unpacking of nucleic acids in cytosol are two major challenges for efficient gene delivery. Herein, we report a polymeric gene delivery vector, which consists of a photosensitizer (PS) with aggregation-induced emission (AIE) characteristics and oligoethylenimine (OEI) conjugated via an aminoacrylate (AA) linker that can be cleaved by reactive oxygen species (ROS). In aqueous media, the polymer could self-assemble into bright red fluorescent nanoparticles (NPs), which can efficiently bind to DNA through electrostatic interaction for gene delivery. Upon visible light irradiation, the generated ROS can break the endo/lysosomal membrane and the polymer, resulting in light-controlled endo/lysosomal escape and unpacking of DNA for efficient gene delivery. The smart polymer represents the first successful gene vector to simultaneously address both challenges with a single light excitation process.
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