期刊
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
卷 47, 期 5, 页码 604-606出版社
WALTER DE GRUYTER GMBH
DOI: 10.1515/CCLM.2009.138
关键词
antibody interference; electrochemiluminescent immunoassay (ECLIA); polyethylene glycol (PEG); thyroid-stimulating hormone (TSH)
Background: Three adult patients presented with unexpectedly high thyrotropin (TSH) concentrations that were discordant with clinical and biochemical findings of euthyroid or hyperthyroid status. Methods: Antibody interference in the TSH immunoassay (Roche) was investigated by polyethylene glycol (PEG)-pretreatment, heterophilic blocking tube (HBT)-pretreatment, rheumatoid factor (RF) testing, immunofixation, protein A adsorption, and gel filtration chromatography (GFC). Results: PEG-precipitation yielded <20% recovery of serum TSH, whereas HBT-pretreatment did not decrease TSH test results. RF-testing and immunofixation were negative. Protein A adsorption and GFC demonstrated the presence of TSH-immunoglobulin complexes in serum. Conclusions: Interference by TSH-immunoglobulin complexes should be ruled out in euthyroid and hyperthyroid patients presenting with inappropriately increased or non-suppressed TSH values. Clin Chem Lab Med 2009;47:604-6.
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