期刊
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷 38, 期 10, 页码 1052-1060出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10826076.2015.1012522
关键词
reactive oxygen species; lipid peroxidation; endogenous antioxidants; electrochemical detector; hyperlipidemia; high-performance liquid chromatography
资金
- Higher Education Commission of Pakistan
A new method has been developed and validated based on high-performance liquid chromatography for simultaneous determination of endogenous aminothiols (cystine, cysteine, homocystiene, methionine, reduced and oxidized glutathione, N-acetylcysteine), ascorbic acid, and malondialdehyde in single elution using electrochemical detector kept at 900mV electrode potential on Discovery 250mm C18 RP chromatographic column. The elution time was 20min, using flow rate of 0.6mL/min, injecting 5 mu L sample for analysis after extraction. The method was validated according to standard guidelines for accuracy, linearity, specificity, reproducibility, limit of detection and limit of quantification and was successfully applied to serum samples of healthy animal model. The analytes were determined using dilution method and values were calculated in mmol/L. This method can be applied to clinical pathologies related to oxidative stress and metabolic syndromes for assessment of severity and facilitate in developing a correlation between the endogenous antioxidant capacities with exogenous antioxidant interventions.
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