期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 10, 页码 3347-3351出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201508978
关键词
cancer; DNA; RNA nanotechnology; multifunctionality; RNA interference; siRNA delivery
资金
- DoD OCRP Teal Innovator Award [OC120504]
- Koch Institute Core Grant from the NCI [P30-CA14051]
- National Research Foundation of Korea [2015R1C1A1A02037770]
- Yonsei University Future-leading Research Initiative [2015-22-0085]
- National Institutes of Health [1F32EB017614-02]
- National Research Foundation of Korea [2015R1C1A1A02037770] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- CDMRP [OC120504, 542483] Funding Source: Federal RePORTER
Packaging multiple small interfering RNA (siRNA) molecules into nanostructures at precisely defined ratios is a powerful delivery strategy for effective RNA interference (RNAi) therapy. We present a novel RNA nanotechnology based approach to produce multiple components of polymerized siRNA molecules that are simultaneously self-assembled and densely packaged into composite sponge-like porous microstructures (Multi-RNAi-MSs) by rolling circle transcription. The Multi-RNAi-MSs were designed to contain a combination of multiple polymeric siRNA molecules with precisely controlled stoichiometry within a singular microstructure by manipulating the types and ratios of the circular DNA templates. The Multi-RNAi-MSs were converted into nanosized complexes by polyelectrolyte condensation to manipulate their physicochemical properties (size, shape, and surface charge) for favorable delivery, while maintaining the multifunctional properties of the siRNAs for combined therapeutic effects. These Multi-RNAi-MS systems have great potential in RNAi-mediated biomedical applications, for example, for the treatment of cancer, genetic disorders, and viral infections.
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