期刊
CLINICAL CANCER RESEARCH
卷 14, 期 12, 页码 3832-3839出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/1078-0432.CCR-07-5067
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- NCRR NIH HHS [U24 RR021760] Funding Source: Medline
- NIBIB NIH HHS [R01 EB000993, R01 EB000993-01A1] Funding Source: Medline
- NINDS NIH HHS [F32 NS058164-01A1, F32 NS058164, 1F32NS058164-01] Funding Source: Medline
Purpose: Clinical trials have commenced to evaluate the feasibility of targeting malignant gliomas with genetically engineered CTLs delivered directly to the tumor bed in the central nervous system. The objective of this study is to determine a suite of magnetic resonance imaging (MRI) measurements using an ortholopic xenograft murine model that can noninvasively monitor immunologically mediated tumor regression and reactive changes in the surrounding brain parenchyma. Experimental Design: Our preclinical therapeutic platform is based on CTL genetic modification to express a membrane tethered interleukin-13 (IL-13) cytokine chimeric T-cell antigen receptor. This enables selective binding and signal transduction on encountering the glioma-restricted IL-13 alpha 2 receptor (IL-13R alpha 2). We used MRI to visualize immune responses following adoptive transfer of IL-13R alpha 2-specific CD8(+) CTL clones. Results: Based on MRI measurements, several phases following IL-13R alpha 2-specific T-cell adoptive transfer could be distinguished, all of which correlated well with glioblastoma regression confirmed on histology. The first detectable changes, 24 hours post-treatment, were significantly increased T-2 relaxation times and strongly enhanced signal on T-1-weighted postcontrast images. In the next phase, the apparent diffusion coefficient was significantly increased at 2 and 3 days post-treatment. In the last phase, at day 3 after IL-13R alpha 2-specific T-cell injection, the volume of hyperintense signal on T-1-weighted postcontrast image was significantly decreased, whereas apparent diffusion coefficient remained elevated. Conclusions: The present study indicates the feasibility of MRI to visualize different phases of immune response when IL-13R alpha 2-specific CTLs are administered directly to the glioma tumor bed. This will further the aim of better predicting clinical outcome following immunotherapy.
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