期刊
CLINICAL BIOCHEMISTRY
卷 44, 期 13, 页码 1050-1057出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2011.06.004
关键词
Piwi-interacting RNA; Gastric cancer; Circulating tumor cells; Gene diagnosis
资金
- Natural Science Foundation of Ningbo [2010A610044]
- Zhejiang Provincial Research Project [2010C33112]
- Natural Sciences Foundation of Zhejiang [Y207240]
- National Natural Science Foundation of China [30872420]
- Scientific Research Foundation of the Graduate School of Ningbo University [G10JA026]
- Excellent Dissertation Fund of Ningbo University [201014]
- K. C. Wong Magna Fund in Ningbo University
Objective: To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers. Design and methods: Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in the peripheral blood of 93 patients with gastric cancer and 32 healthy volunteers. Receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic values. Results: The peripheral blood levels of piR-651 and piR-823 in the patients with gastric cancer were significantly lower than those from controls (P<0.001). The piR-651 level in gastric adenocarcinoma was higher than that in gastric signet ring cell carcinoma (P=0.003). The piR-823 level was positively associated with tumor-node-metastasis stage (P=0.027) and distant metastasis (P=0.026). The areas under the ROC curve were 0.841, 0.812 and 0.860 for piR-651, piR-823 and the combination, respectively. Conclusions: piRNAs may be valuable biomarkers for detecting circulating gastric cancer cells. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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