期刊
CLINICAL BIOCHEMISTRY
卷 44, 期 10-11, 页码 864-868出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.clinbiochem.2011.04.020
关键词
Vitamin D; High performance liquid chromatography; Radio immunoassay; Methodology
Objective: To compare the performance of radioimmunoassay (RIA) with high-performance liquid chromatography (HPLC) and electrochemiluminescence (ECLIA) for the quantification of vitamin D (25OHD). Methods: HPLC method for the determination of 25OHD in human biological samples was developed and compared in terms of accuracy and precision with a commercially available RIA assay. Performance of RIA assay with ECLIA technology for 25OHD analysis was further compared. Results: Median 25OHD levels with HPLC vs. RIA were 50.1 nmol/L (IQ= 17.7-199.4 nmol/L) and 51.1 nmol/L (IQ= 12.5-187.2 nmol/L) respectively, whereas median 25OHD concentration with RIA vs. ECLIA was 32.4 nmol/L (9.98-199.7 nmol/L) and 29.9 nmol/L (4.9-214.6 nmol/L), respectively. Comparison data for HPLC vs. RIA showed RIA= 1.13 + 1.01 (HPLC) (RMSE = 11.2 nmol/L) and for RIA vs. ECLIA revealed, ECLIA = 3.21 + 0.9 (RIA) (RMSE9.6 nmol/L). Conclusion: Acceptable correlation was observed among HPLC and RIA and also with RIA and ECLIA in quantification of 25OHD. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据