期刊
CLINICAL AND VACCINE IMMUNOLOGY
卷 18, 期 1, 页码 43-49出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/CVI.00292-10
关键词
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资金
- Bill and Melinda Gates Foundation
- NIH [P30 AI051445, UC6 AI058607, P30 AI64518, S10RR019145]
- NATIONAL CENTER FOR RESEARCH RESOURCES [S10RR019145] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [P30AI064518, P30AI051445, UC6AI058607] Funding Source: NIH RePORTER
Recombinant mycobacteria hold promise as vectors for delivery of HIV-1 and other pathogen antigen inserts for inducing systemic and mucosal immune responses. In general, the immunogenicity of the recombinant mycobacterial insert is proportional to the level of insert expression. In this study, a novel flow cytometry-based assay has been developed to sort live recombinant mycobacterial mutants with high expression of foreign inserts and to enrich those sorted bacterial populations. Sorted recombinant mycobacterial clones expressed higher levels of the ovalbumin SIINFEKL epitope, and select sorted clones showed better immunogenicity than unsorted recombinant mycobacteria. Thus, flow cytometry-based sorting can isolate recombinant mycobacteria enriched for higher insert expression.
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