期刊
CLINICAL AND EXPERIMENTAL MEDICINE
卷 11, 期 2, 页码 105-112出版社
SPRINGER-VERLAG ITALIA SRL
DOI: 10.1007/s10238-010-0110-9
关键词
Glioma; Wnt/beta-Catenin; Cyclin D1; Immunohistochemical staining; Prognosis
资金
- Social Development Foundation of Jiangsu, China [BS2007037]
- Medicine, Jiangsu Province Key Talent Foundation [RC2007029]
- Science and Technology Development Foundation of Huaian, China [HAS07025]
Gliomas are the most common primary intracranial tumors. Understanding the molecular basis of gliomas' progression is required to develop more effective therapies. The Wnt/beta-catenin signaling cascade is an important signal transduction pathway in human cancers. Although, overactivation of this pathway is a hallmark of several forms of cancer, little is known about its role in human gliomas. Here, we aimed to determine the clinical significance of Wnt/beta-catenin pathway components in gliomas. Immunohistochemical staining was performed to detect the expression patterns of Wnt1, beta-catenin and Cyclin D1 in the biopsies from 96 patients with primary gliomas. Kaplan-Meier survival and Cox regression analyses were performed to evaluate the prognosis of patients. Cytoplasmic staining pattern of Wnt1, membranous, cytoplasmic and nuclear accumulation of beta-catenin, and nuclear localization of Cyclin D1 were demonstrated by immunohistochemical staining. The Wnt1 expression significantly correlated with the expression of Cyclin D1 (P < 0.0001). The ratio of tumors with a cytoplasmic-nuclear pattern or a cytoplasmic pattern of beta-catenin was significantly higher in Wnt1-positive (P < 0.01) and Cyclin D1-positive (P < 0.01) tumors than in Wnt1-negative and Cyclin D1-negative tumors, respectively. The protein expression levels of Wnt1, beta-catenin and Cyclin D1 were all positively correlated with the Karnofsky performance scale (KPS) score and World Health Organization (WHO) grades of patients with gliomas. Furthermore, Wnt1, cytoplasmic-nuclear beta-catenin and Cyclin D1 status were all the independent prognostic factors for glioma patients (P = 0.01, 0.007 and 0.005, respectively). These results provide convincing evidence that the Wnt/beta-catenin pathway correlated closely with the progression of gliomas and might be a novel prognostic marker for this neoplasm.
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