期刊
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
卷 179, 期 1, 页码 50-61出版社
WILEY
DOI: 10.1111/cei.12344
关键词
chemotaxis; cytokines; inflammation; macrophage; phagocytosis
类别
资金
- European Commissions (DoReMi, European Network of Excellence) [249689]
- German Research Foundation [GA 1507/1-1, SFB914]
- German Federal Ministry of Education and Research (GREWIS) [02NUK017G]
Benign painful and inflammatory diseases have been treated for decades with low/moderate doses of ionizing radiation (LD-X-irradiation). Tissue macrophages regulate initiation and resolution of inflammation by the secretion of cytokines and by acting as professional phagocytes. Having these pivotal functions, we were interested in how activated macrophages are modulated by LD-X-irradiation, also with regard to radiation protection issues and carcinogenesis. We set up an ex-vivo model in which lipopolysaccharide pre-activated peritoneal macrophages (pM phi) of radiosensitive BALB/c mice, mimicking activated macrophages under inflammatory conditions, were exposed to X-irradiation from 001 Gy up to 2 Gy. Afterwards, the viability of the pM phi, their transmigration and chemotaxis, the phagocytic behaviour, the secretion of inflammatory cytokines and underlying signalling pathways were determined. Exposure of pM phi up to a single dose of 2 Gy did not influence their viability and phagocytic function, an important fact regarding radiation protection. However, significantly reduced migration, but increased chemotaxis of pM phi after exposure to 01 or 05 Gy, was detected. Both might relate to the resolution of inflammation. Cytokine analyses revealed that, in particular, the moderate dose of 05 Gy applied in low-dose radiotherapy for inflammatory diseases results in an anti-inflammatory cytokine microenvironment of pM phi, as the secretion of the proinflammatory cytokine interleukin (IL)-1 was reduced and that of the anti-inflammatory cytokine transforming growth factor (TGF)- increased. Further, the reduced secretion of IL-1 correlated with reduced nuclear translocation of nuclear factor (NF)-B p65, starting at exposure of pM phi to 05 Gy of X-irradiation. We conclude that inflammation is modulated by LD-X-irradiation via changing the inflammatory phenotype of macrophages.
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