期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 9, 页码 2806-2810出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201410573
关键词
bioorthogonal labeling; biosensors; glycosylation; host-guest systems; xenon
资金
- European Research Council under the European Community/ERC [242710]
- Leibniz Association (WGL) [SAW-2011-FMP-2]
- Human Frontier Science Program [LT000858/2010]
- DFG [SFB 765, SPP1623]
- Fonds der Chemischen Industrie
- Einstein Foundation
- Boehringer-Ingelheim Foundation (Plus 3 Award)
The targeting of metabolically labeled glycans with conventional MRI contrast agents has proved elusive. In this work, which further expands the utility of xenon Hyper-CEST biosensors in cell experiments, we present the first successful molecular imaging of such glycans using MRI. Xenon Hyper-CEST biosensors are a novel class of MRI contrast agents with very high sensitivity. We designed a multimodal biosensor for both fluorescent and xenon MRI detection that is targeted to metabolically labeled sialic acid through bioorthogonal chemistry. Through the use of a state of the art live-cell bioreactor, it was demonstrated that xenon MRI biosensors can be used to image cell-surface glycans at nanomolar concentrations.
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