期刊
CLINICAL & EXPERIMENTAL METASTASIS
卷 30, 期 5, 页码 615-630出版社
SPRINGER
DOI: 10.1007/s10585-013-9565-x
关键词
Intratumor heterogeneity; Invasion; Migration; 3D co-culture; Extracellular matrix
类别
资金
- Cornell Center on the Microenvironment & Metastasis from the National Cancer Institute (NCI) [U54CA143876]
- NSF [CMMI-1233827]
- National Science Foundation
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1233827] Funding Source: National Science Foundation
Solid tumors consist of genetically and phenotypically diverse subpopulations of cancer cells with unique capacities for growth, differentiation, and invasion. While the molecular and microenvironmental bases for heterogeneity are increasingly appreciated, the outcomes of such intratumor heterogeneity, particularly in the context of tumor invasion and metastasis, remain poorly understood. To study heterotypic cell-cell interactions and elucidate the biological consequences of intratumor heterogeneity, we developed a tissue-engineered multicellular spheroid (MCS) co-culture model that recapitulates the cellular diversity and fully three-dimensional cell-cell and cell-matrix interactions that characterize human carcinomas. We found that invasion-competent malignant cells induced the collective invasion of otherwise invasion-incompetent epithelial cells, and that these two cell types consistently exhibited distinct leader and follower roles during invasion. Analysis of extracellular matrix (ECM) microarchitecture revealed that malignant cell invasion was accompanied by extensive ECM remodeling including matrix alignment and proteolytic track-making. Inhibition of cell contractility- and proteolysis-mediated matrix reorganization prevented leader-follower behavior and malignant cell-induced epithelial cell invasion. These results indicate that heterogeneous subpopulations within a tumor may possess specialized roles during tumor progression and suggest that complex interactions among the various subpopulations of cancer cells within a tumor may regulate critical aspects of tumor biology and affect clinical outcome.
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