期刊
CLINICAL & EXPERIMENTAL METASTASIS
卷 29, 期 6, 页码 625-637出版社
SPRINGER
DOI: 10.1007/s10585-012-9476-2
关键词
Melanoma; Chemokines; Chemokine receptors; Metastasis; CXCL12; CXCR4; CCL27; CCR10; Prognosis
类别
资金
- Instituto de Salud Carlos III-Subdireccion General de Evaluacion y Fomento de la Investigacion [PI071203, PI070805]
- Conselleria de Educacion,Generalitat Valenciana [PROMETEO/2011/084]
- Fundacion parael Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana-Fundacion Bancaja (IMPULSA), Spain
CXCR4, CCR7 and CCR10 chemokine receptors are known to be involved in melanoma metastasis. Our goal was to compare the relative intratumoral mRNA expression of these receptors with that of their corresponding chemokine ligands, CXCL12, CCL19, CCL21, and CCL27 across the full spectrum of human melanoma progression: thin and thick primary melanomas, as well as in transit, lymph node, and distant metastases. Expression was quantified by real-time RT-PCR in 103 melanoma samples: 51 primary tumors and 52 metastases. Particular emphasis was focused on chemokine ligand-receptor expression ratios. Immunohistochemistry was performed to identify the cell types expressing these molecules. CXCL12-CXCR4 and CCL27-CCR10 ratios were higher in thin than in thick primary melanomas, and all four chemokine-receptor ratios were higher in primary tumors than in melanoma metastases. CCL27-CCR10 and CXCL12-CXCR4 expression ratios in primary tumors were inversely associated with the development of distant metastases, and improved the predictive value of tumor thickness for distant metastasis, which is important since chemokine ligand-receptor ratios are not affected by the endogenous gene employed for normalizing mRNA expression. Both receptor and ligand immunolabeling were detected in neoplastic cells suggesting autocrine mechanisms. Our results support the concept that low CCL27/CCR10 and CXCL12/CXCR4 intratumoral mRNA ratios are associated with melanoma progression, and in combination with Breslow thickness, are the best predictive factors for the development of distant metastases in primary cutaneous melanoma.
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