期刊
CLINICAL & DEVELOPMENTAL IMMUNOLOGY
卷 -, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2012/350209
关键词
-
类别
资金
- Natural Science Foundation of China [30872335, 81172871]
- Medical Science and Technology Development Foundation of Jiangsu Province Department of Health [H200950]
- Jiangsu Government
It has been reported that Notch family proteins are expressed in synovium tissue and involved in the proliferation of synoviocyte from rheumatoid arthritis (RA). The aim of this paper was to investigate whether Notch signaling mediated TNF-alpha-induced cytokine production of cultured fibroblast-like synoviocytes (FLSs) from RA. Exposure of RA FLSs to TNF-alpha (10 ng/ml) led to increase of Hes-1, a target gene of Notch signaling, and a marked upregulation of Notch 2, Delta-like 1, and Delta-like 3 mRNA levels. Blockage of Notch signaling by a.-secretase inhibitor (DAPT) inhibited IL-6 secretion of RA FLSs in response to TNF-alpha while treatment with recombinant fusion protein of Notch ligand Delta-like 1 promoted such response. TNF-alpha stimulation also induced IL-6 secretion in OA FLSs; however, the Hes-1 level remained unaffected. Our data confirm the functional involvement of Notch pathway in the pathophysiology of RA FLSs which may provide a new target for RA therapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据