期刊
CLINICA CHIMICA ACTA
卷 433, 期 -, 页码 69-75出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cca.2014.02.019
关键词
CD45-FISH; ICC; Lung cancer; Ovarian tumor; CTCs
资金
- Beijing Municipal Science & Technology Commission [Z121107005112002]
- Young Scientific Research Fund of Peking Union Medical College Hospital
Background: Conventional cytokeratin (CK)-based methods to detect circulating tumor cells (CTCs) often present suboptimal sensitivities for decreased or non-expression of cytokeratin in some CTCs. We clinically investigated a new method that combines immunocytochemistry staining (ICC) of CD45 and fluorescence in situ hybridization (FISH). Methods: Circulating epithelial cells from 141 subjects were enriched using an EpCAM-independent strategy and then identified by either a combination of FISH with chromosome 8 centromere probe (CEP8) and ICC staining of CD45 (CD45-FISH) or ICC staining of CK Results: For detecting CTCs enriched from lung cancers, CD45-FISH had larger areas under ROC curves of 0.963 (P = 0.000) compared to ICC (0.653; P = 0.031) using cut-off values of 2 and 1 cell/3.75 ml blood with sensitivities of 83.3% and 43.3%, specificities of 98.6% and 89.5%, respectively. Moreover, CD45-FISH showed 76.2% sensitivity in detecting CTCs in ovarian cancers (P < 0.001). Four of six ovarian cancers showed dramatical decrease in both CTCs and serum CA125 on the 7th day after surgery. Conclusion: CD45-FISH method had improved sensitivity and specificity in detecting CTCs of lung and ovarian cancers compared to ICC-CK. This combined detection strategy may be useful in detecting or monitoring CTCs after ovarian cancer surgery. (C) 2014 Elsevier B.V. All rights reserved.
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