期刊
CLINICA CHIMICA ACTA
卷 411, 期 17-18, 页码 1361-1366出版社
ELSEVIER
DOI: 10.1016/j.cca.2010.05.038
关键词
LDL subclass phenotype; Small dense LDL-cholesterol; Triglycerides; Apolipoprotein B; Apolipoprotein A-I; Non-HDL-cholesterol
资金
- Belgian Lipid Club (BLC)
Background: A new method (sLDL-EX SEIKEN) is commercially available for the direct quantification of small dense LDL-cholesterol (sd-LDL) on automated chemistry analyzers, without manual sample pretreatment. We evaluated the performance of this direct assay to estimate the small dense LDL subclass phenotype (non-A), defined by polyacrylamide gel tube electrophoresis. Methods: Fasting serum samples from 189 healthy subjects (age 18-75y, 96 females) were collected. The direct sd-LDL assay (from Randox Laboratories) was applied on a Roche Modular P analyzer. The Quantimetrix Lipoprint (TM) LDL System was used to define LDL subclass phenotypes (A and non-A). ROC curve analysis was performed for sd-LDL and other lipids and apolipoproteins (apo) with respect to phenotype non-A. Results: sd-LDL concentrations (40.4 +/- 18.6 mg/dl) in the total group correlated (P<0.0001) with apoB (r = 0.831), apoB/A-I ratio (r=0.757), non-HDL-cholesterol (r=0.821), triglycerides (r=0.439), and LDL-cholesterol (r= 0.641). Higher sd-LDL concentrations (P<0.0001) were measured in subjects with LDL phenotype non-A (53.6 +/- 17.0 mg/dl, n = 92) than in those with phenotype A (27.9 +/- 8.9 mg/dl, n = 97). In logistic regression analysis, sd-LDL and apoA-I were independently associated with LDL subclass phenotype non-A. Highest areas under ROC curves were obtained for sd-LDL (0.943), triglycerides (0.833), triglyceride/HDL-cholesterol (0.838) and apoB/A-I ratio (0.826) to predict phenotype non-A. The sd-LDL cut-off point for optimal sensitivity (87.9%) and specificity (92.8%) was >38.5 mg/dl. Conclusions: The direct, homogeneous sd-LDL method is easily applicable on an automated chemistry analyzer and shows acceptable performance to estimate the electrophoretic LDL subclass phenotype. (C) 2010 Elsevier B.V. All rights reserved.
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