期刊
CLINICA CHIMICA ACTA
卷 411, 期 5-6, 页码 336-344出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.cca.2009.11.023
关键词
iTRAQ; Lipoproteome; Low-density lipoprotein; Quantitative proteomics; Two-dimensional electrophoresis; Very-low-density lipoprotein
资金
- National Science Council of Taiwan [NSC 95-2320-B-006-021, NSC 96-2320-B-006-005]
- National Cheng Kung University [B03S]
Background: Low-density lipoprotein (LDL) is a natural metabolite of very-low-density lipoprotein (VLDL) in the circulation. Systematic investigation of total protein components and dynamics might provide insights into this normal metabolic process. Methods: VLDL and LDL were purified from normolipidemia pooled plasma by gradient ultracentrifugation with either ionic or non-ionic media. The protein contents were compared by liquid chromatography tandem mass analyses based on isobaric tag for relative and absolute quantitation and two-dimensional gel electrophoresis. Results: Our comparative lipoproteomes revealed 21 associated proteins. Combined with Western blot analysis, and on the basis of the differential expression levels we classified them into 3 groups: (i) VLDL>LDL [apolipoprotein (apo) A-IV, apo(a), apoCs, apoE, apoJ and serum amyloid A-4]; (ii) VLDL
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